The 13C/12C and 15N/14N ratios of 572 individual Skeletonema cells and of 306 individual Chaetoceros cells were determined for samples incubated with 13C and 15N tracers, whereas 13C/12C and 15N/14N ratios of 102 cells were determined from incubations without isotope tracers (controls). C and N content of diatom cells were calculated from their dimensions measured by light microscopy in parallel samples28. Isotope ratio data were extracted from individual cells based on cell morphology visualised in the 12C14N ion images. Triplicate subsamples for isotope analysis were transferred into 12 mL Exetainer©vials and biological activity stopped by adding 100 µL 50% ZnCl2 solution. Isotopic ratios of 28N2, 29N2 and 30N2 were analysed by gas chromatography isotope ratio mass spectrometry on a Thermo Delta V isotope ratio mass spectrometer. The small interference by 11B16O isotopes from the silicate frustule at a mass resolution of 6000 (M/ΔM) was corrected for when subtracting the natural apparent signal from 13C/12C in un-amended samples (controls) from that of amended samples. A natural bond orbital (NBO) analysis28 of 5 indicated that the positive charge is distributed over the H atoms (formal charge on H1: 0.237; H2: 0.447), including those of the CH3 group, rather than being localized on the N atom (−0.688) (Supplementary Fig. S2).

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Beginning on June 15 at Cherbourg, in Operation Ariel, Allies spirited soldiers away from Saint-Malo, Brest, Saint-Nazaire, and other ports all the way down the French coast to the border with Spain. Because the balls are metal, this operation generates a lot of heat, so water pours over the balls and plates to cool them. These results suggest that the results in the gas phase should at least qualitatively correlate with those in water or a ligand-binding pocket, as the latter does generally not contain many water molecules. These results indicate that the nitrogen cation is important for the formation of the C-H· C-H···O hydrogen bonds are low relative to those of heteroatom-hydrogen bonds, π/π interactions, cation/π interactions, or CH/π interaction models (Supplementary Fig. S5). C-H···O hydrogen bonds to those of interactions which are often considered in drug design. C-H···O hydrogen bond models, considering that the electrostatic force controls the geometry of the heteroatom-hydrogen bonds27. C-H··O hydrogen bond models. C-H···O hydrogen bonds, and examined the dependence of their interaction energy on the geometry. C-H···O hydrogen bonds remains low, especially regarding the character of the hydrogen acceptor oxygen atoms and the relationship between the energy and the geometry of these interactions.

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